Immune Receptor Profiling Nanocourse- June 1, 2018

All course materials can be accessed at https://github.com/initoby/VDJServer_Nanocourse

AGENDA

Contacts

Time Topic
8:45am – 9:00am Setup, Registration, provide workshop handouts to participants
9:10am – 10:30am Introduction session
9:10am – 10:30am Immune system overview (including immune sequencing technology and summary of vdjserver workflow for analysis); Setup IgBlast jobs with demo files
Show slides and video
10:30am-10:40am Break
10:40am – 11:45am Session 1- Data digest
Demo data and VDJServer hands-on demonstrations
Elaborate on the functionalities and benefits of VDJServer
Breakout and setup RepCalc VDJServer “jobs” (provide one-on-one assistance and point participants to quickstart protocol @ handout pages)
11:45am – 1:00pm Lunch
1:00pm – 2:40pm Session 2- Community standards, Data analysis and interpretation
Example of pre-processing using example fastq files (vdjserver project)
AIRR community and MiAIRR Standards
Analysis and interpretation of repertoire calculations output
2:40pm-2:55pm Break
3:00pm-3:45pm Downstream application of VDJServer products in machine learning (example dataset using the demo dataset and discussion of some outcomes)
3:45pm-4:30pm Summarize the workshop and encourage future interactions
Invitation to use some example datasets for further analysis (VDJServer team will assist in setup as time permits)